Detection of Babesia ovis Using Polymerase Chain Reaction
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چکیده
A procedure was developed for delection of Babesia ovis (B.ovis) infection in blood samples containing infected erythrocytes by polymerase chain reaction (l'CR). In order to deteet the DNA parasite in blood sample two sets of oligonucleotides were designed according to the nucleotide sequences of the l8S rRNA and ATP-binding prote in genes of H ovis. These genes were amplified at 286 and 453bp fragments. respectively. Samples l'rom eight isolates of experimentally infected lambs to B.ovis were analyzed. PCR analysis showed that ail of the samplcs were inCected by Hovis. Sequence determination and analysis of amplified fragments confirmed speeificity of the l'CR. The PCR was sensitive enough to detect parasite DNA from 51ll of blood samples \Vith a parasitemia of 0.000005%. These results suggest that the PCR-based diagnostic assay for Havis is highly specific and sensitive.
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تاریخ انتشار 2017